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R. Saranya ¹, K. Tamilarasan ¹, N. Peatciyammal ¹, Rajamaniccam L. Sai ², M. Dharmendira Kumar ²

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai 600069, IN
2 Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai 600025, IN

Abstract

A Response Surface approach has been used to study the production of an extracellular lipase from Bacillus species. This research paper mainly focused on developing a media component and physical parameters for lipase production by Central Composite Design (CCD).Interaction were studied for four different factors are olive oil concentration, ammonium sulphate concentration, pH and Temperature. By using the statistical approach (Response Surface Methodology) the optimum enzyme activity of 8.69 IU/ml was predicted when olive oil concentration 1.5%, ammonium sulphate concentration 0.4%, pH 8, Temperature 40ºC.

Keywords

Central Composite Design, Lipase Activity, Maximum Yield, Response Surface Methodology, Statistical Approach.

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S. R. Yamunarani ¹, M. Dinesh kumar ¹, B. Balachander ¹, K. Tamilarasan ¹, C. Muthukumaran ²

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai -600069, IN
2 Department of Biotechnology, SRM University, Kattankulathur, Chennai -603203, IN

Abstract

This paper mainly focused to study and optimizes the parameters like carbon sources, substrate concentration, pH, Temperature for the maximum production of amylase by Aspergillus oryzae MTCC 1847. Different Carbon sources such as Sweet potato, Rice bran and Wheat Bran were to be used as the substrate for the enzyme production. The enzyme activity was observed high when the medium contained Sweet Potato as Carbon substrate with 2% concentration, pH of 6.5, Temperature of 35°C.

Keywords

Sweet Potato, Rice Bran, Wheat Bran, Aspergillus oryzae.

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